Papers

Rational design of disordered proteins for sequence–function investigation

K Hunter, T Brandt, K Guadalupe, K.C. Kolamunna, J.M. Lotthammer, N.M. Shamoon, J.K. Niblo, B Nichols, L.M. Day, A Martinez, A.S. Holehouse, S Sukenik, R.J. Emenecker

Nature. Published online July 29, 2026

Intramolecular interactions between folded and disordered regions shape ubiquilin structure and function

J.K. Niblo, N Acharya, M.B. Watkins, C.A. Castañeda, S Sukenik

Advanced Science. 2026; 13(48):e75904

Infection tunes the dynamics of adenoviral E1A disordered regions

P Koenig, A Truong, H Lehman, B.-J. Sanchez, J.A. Grasis, S Sukenik

bioRxiv 2026.04.12.717990

STI1 domain engages transient helices to mediate Dsk2 phase separation and proteasome condensation

N Acharya, E.A. Daniel, T.P. Dao, J.K. Niblo, E.O. Mulvey, S Sukenik, D.A. Kraut, J Roelofs, C.A. Castañeda

The EMBO Journal. 2026; 45(8):2712-2738

A geothermal amoeba sets a new upper temperature limit for eukaryotes

H.B. Rappaport, N.A. Petek-Seoane, T Tyml, F Mikus, K LaButti, G Ani, J.K. Niblo, E MacVicar, R.M. Shepherd, I de la Higuera, S.J. Lord, G Dey, G.V. Wolfe, O Dudin, S Sukenik, L.A. Katz, K.M. Stedman, K Skruber, F Schulz, R.D. Mullins, A.M. Oliverio

bioRxiv 2025.11.24.690213

OsmoFold: A high-throughput tool for predicting the impact of osmolytes on protein structure

V Nicholson, S Maharjan, S Sukenik, T.C. Boothby

Biophysical Journal. 2025; 124(21):3555-3570

Protein surface chemistry encodes an adaptive tolerance to desiccation

P.S. Romero-Pérez, H.M. Moran, D.P. Cordone Jr., A Horani, A Truong, E Manriquez-Sandoval, J.F. Ramirez, A Martinez, E Gollub, K Hunter, K.C. Kolamunna, J.M. Lotthammer, R.J. Emenecker, H Liu, J.H. Iwasa, T.C. Boothby, A.S. Holehouse, S.D. Fried, S Sukenik

Cell Systems. 2025; 16(10):101407

A phase transition modulates the protective function of a tardigrade disordered protein during desiccation

K Nguyen, S Biswas, S Kc, A Walgren, V Nicholson, C Childs, B.X. Medina-Rodriguez, V Alvarado, S Sukenik, A.S. Holehouse, T.C. Boothby

Protein Science. 2025; 34(10):e70300

Functional diversity of Arabidopsis late embryogenesis abundant proteins in response to changes in the physicochemical environment

L.D. Palomino-Navarrete, D Pérez-Villanueva, D Moses, E Gollub, K Nguyen, S Sanchez-Martinez, F Yu, K Martinez, T.C. Boothby, S Sukenik, C.L. Cuevas-Velazquez

bioRxiv 2025.08.26.672499

Single-point mutations in disordered proteins: Linking sequence, ensemble, and function

E Flores, N Acharya, C.A. Castañeda, S Sukenik

Current Opinion in Structural Biology, Volume 91, 102987

Correlating disordered activation domain ensembles with gene expression levels

E Flores, A.R. Camacho, E Cuevas-Zepeda, M.B. McCoy, F Yu, M.V. Staller, S Sukenik

Biophysical Reports, Volume 5, Issue 1, 100195

LEA_4 motifs function alone and in conjunction with synergistic cosolutes to protect a labile enzyme during desiccation

V Nicholson, K Nguyen, E Gollub, M McCoy, F Yu, A.S. Holehouse, S Sukenik, T.C. Boothby

Protein Science. 2025; 34(2):e70028

Protein surface chemistry encodes an adaptive tolerance to desiccation

P.S. Romero-Pérez, H.M. Moran, A Horani, A Truong, E Manriquez-Sandoval, J.F Ramirez, A Martinez, E Gollub, K Hunter, et al.

Disordered proteins interact with the chemical environment to tune their protective function during drying

KC Shraddha, K.H. Nguyen, V Nicholson, A Walgren, T Trent, E Gollub, P.S. Romero-Perez, A.S. Holehouse, S Sukenik, T.C. Boothby

eLife 13:RP97231

YAP condensates are highly organized hubs

S Hao, Y.J. Lee, N.B. Goldfajn, E Flores, et al.

iScience, Volume 27, Issue 6, 109927

Structural biases in disordered proteins are prevalent in the cell

D Moses, K Guadalupe, F Yu, E Flores, A Perez, R McAnelly, N M Shamoon, E Cuevas-Zepeda, A Merg, E W. Martin, A S. Holehouse, S Sukenik

Nat Struct Mol Biol 31, 283–292 (2024)

Helicity of a tardigrade disordered protein contributes to its protective function during desiccation

S Biswas, E Gollub, F Yu, G Ginell, A Holehouse, S Sukenik, et al.

Protein Science. 2024; 33(2):e4872

Labile assembly of a tardigrade protein induces biostasis

S Sanchez-Martinez... F Yu, E Gollub... S Sukenik... TC Boothby

Protein Science. 2024; 33(4):e4941

Intrinsically disordered regions are poised to act as sensors of cellular chemistry

David Moses, Garrett M. Ginell, Alex S. Holehouse, Shahar Sukenik

Trends Biochem. Sci

Helicity of a tardigrade disordered protein promotes desiccation tolerance

Sourav Biswas, Edith Gollub, Feng Yu, Garrett Ginell, Shahar Sukenik, Thomas C. Boothby

Protein Science

When Phased Without Water: Biophysics of Cellular Desiccation, from Biomolecules to Condensates

Paulette Sofia Romero-Perez, Yanniv Dorone, Eduardo Flores, Shahar Sukenik, Steven Boeynaems

Chem. Rev.

Structural preferences shape the entropic force of disordered protein ensembles

Feng Yu, Shahar Sukenik

J. Phys. Chem. B

YAP condensates are highly organized hubs for YAP/TEAD transcription

Siyuan Hao, Hannah Fuehrer, Eduardo Flores, Justin Demmerle, Jennifer Lippincott-Schwartz, Zhe Liu, Shahar Sukenik, Danfeng Kai

Hidden structure in disordered proteins is adaptive to intracellular changes

D Moses, K Guadalupe, F Yu, E Flores, A Perez, R McAnelly, N M Shamoon, E Cuevas-Zepeda, A Merg, E W. Martin, A S. Holehouse, S Sukenik

Preprint

Intrinsically disordered protein biosensor tracks the physical-chemical effects of osmotic stress on cells

Cuevas-Velazquez, C.L., Vellosillo, T., Guadalupe, K. et al.

Nat Commun

Myomegalin regulates Hedgehog pathway by controlling PDE4D at the centrosome

H Peng, J Zhang, A Ya, W Ma, S Villa, S Sukenik, X Ge

Molecular Biology of the Cell

Computational modelling of protein conformational changes-application to the opening SARS-CoV-2 spike

A Kucherova, S Strango, S Sukenik, M Theillard

Journal of Computational Physics

A prion-like protein regulator of seed germination undergoes hydration-dependent phase separation

Y Dorone, S Boeynaems, E Flores,... S Sukenik, A D Gitler, S Y Rhee

Cell